I don’t know why you asked this but I sure like the question.
Here’s what I found, according to Rebecca Seal, a neuroscientist at the University of California, San Francisco: (excerpt)
“Neuroscientists now know, however, that it is common for neurons to release a classical transmitter with another type of messenger, such as a gas (nitric oxide, for instance) or a neuropeptide (a small protein that can act as a transmitter). With the aid of new techniques for manipulating and imaging neurons, researchers have found that a number of neurons communicate using more than one classical neurotransmitter. Indeed, some of our auditory neurons simultaneously release three different classical transmitters during a brief period in development.
So we see that “one neuron, one transmitter” is a bit too simplistic. But what about the original principle put forth by Dale that all axonal branches of a neuron release the same transmitter? There now appear to be at least a few exceptions to this principle. Motor neurons, which are important for voluntary muscle movements, have long been known to release acetylcholine onto both muscle cells in the body and neurons in the spinal cord. Recent studies show, however, that motor neurons also release a second transmitter, glutamate. Remarkably, they appear to release glutamate only onto neurons in the spinal cord and not onto muscle cells—in other words, certain branches of a single neuron release glutamate, and others do not.”
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